There are two alternative ways in mushroom spawn manufacturing. First one is based on spores produced by mushrooms on there own and the next method is based on "tissue culture " technique which is more potential on generating identical generation of one particular species.
1.Place a Healthy mature bacidium of the required spc.. as the gills are facing downwards on a clean sterile paper, inside a laminar floor hood .
2. After 20-30 mins. Collect tiny dust-like spores from a sterile inoculating needle and transfer to a sterilized test tube/petri dish filled with distilled water i.e called spore suspension.
3. Mix the suspension with same needle and draw streaks on a pre-prepared PDA medium in a petri dish.
4. Allow 10-12 days of incubation period until it shows charectiristic mycelial growth.
Method 2 : Tissue culture technique
Mushroom spawn production in Sri Lanka is largely based on tisssue culture technique. In here particular tissues from a healthy, characteristic, fresh basidium (mushroom) is separated and artificially grown under controlled conditions in an artificial medium.
Method requires sterilized /autoclaved PDA medium poured in to petri-dishes and cooled atleast for two hours.
1. First, Wipe-off the surface of selected healthy mushroom/basidium with 70% alcohol using cotton wool.
2. Then, remove the unwanted parts of moshroom but keeping the stem-part of the mushroom.
3. Dissect the the basidium and select and separate a tissue from stem-gills intermediate zone of newly cut opened surface.
4. Place the tissue on medium in petri dish . Repeat the step until petri plate consist with around four sections of tissues.
5. Use a parafilm or a cellotape to set two petri dishes firmly well.
6. Place it for 7-14 days incubation until mycelium completely covers the surface of agar medium.
